Myeloperoxidase Chlorination Fluorometric Assay Kit
货号：
10006438-192Well 基本售价：
3066.0 元 规格：
2 x 96 wells
产品信息
概述| 货号 | 10006438-192Well |
| 描述 | Cayman’s Myeloperoxidase Chlorination Fluorometric Assay provides a convenient fluorescence-based method for detecting the MPO chlorination activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3-oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound fluorescein.1 Fluorescein fluorescence is analyzed with an excitation wavelength of 480-495 nm and an emission wavelength of 515-525 nm. The kit includes a MPO-specific inhibitor for distinguishing MPO activity from MPO-independent fluorescence. |
| 别名 | MPO; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Setsukinai, K.i.,Urano, Y.,Kakinuma, K., et al. Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. The Journal of Biological Chemisty 278(5), 3170-3175 (2003).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | 4 |
| 稳定性 | ≥ 6 months |
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