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货号： bs-14046R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-14046R
- 英文名称
- CRB2
- 中文名称
- CRB2蛋白抗体
- 别 名
- Crb2; CRUM2_HUMAN; Crumbs homolog 2 (Drosophila); Crumbs homolog 2; Crumbs-like protein 2; RP11-230L22.2.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 免疫学 神经生物学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 100, 123, 131kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CRB2:701-800/1285 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
CRB2 is a 1,285 amino acid protein that contains three laminin G-like domains and 15 EGF-like domains and exists as multiple alternatively spliced isoforms that are either secreted or membrane bound. Expressed in kidney and retina, as well as in fetal eye and brain, CRB2 is thought to play a role in the morphogenesis of polarized cells and may be involved in DNA repair. Defects in the gene encoding CRB2 are associated with the pathogenesis of autosomal recessive retinitis pigmentosa (RP) and Leber congenital amaurosis (LCA), the former of which refers to a group of diseases that lead to the degeneration of retinal photoreceptor cells, ultimately resulting in a loss of vision. The gene encoding CRB2 maps to human chromosome 9, which houses over 900 genes and comprises nearly 4% of the human genome.
Function:
May play a role in polarized cells morphogenesis.
Subcellular Location:
Secreted and Cell membrane.
Tissue Specificity:
Expressed in retina, fetal eye and brain. Also expressed in kidney, ARPE-19 and RPE/choroid cell lines, and at low levels in lung, placenta, and heart.
Similarity:
Belongs to the Crumbs protein family.
Contains 15 EGF-like domains.
Contains 3 laminin G-like domains.
SWISS:
Q5IJ48
Gene ID:
286204
Database links:Entrez Gene: 286204Human
Entrez Gene: 241324Mouse
Entrez Gene: 366031Rat
Omim: 609720Human
SwissProt: Q5IJ48Human
SwissProt: Q80YA8Mouse
Unigene: 710092Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Eye(Rat) Lysate at 40 ug
Primary: Anti- CRB2 (bs-14046R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 100/123/131 kD
Observed band size: 135 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated(bs-14046R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRB2) Polyclonal Antibody, Unconjugated (bs-14046R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CRB2 Polyclonal Antibody, Unconjugated(bs-14046R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining