SimpleChIP® Human FKBP51 Intron 5 Primers【科瑞奥博】

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SimpleChIP® Human FKBP51 Intron 5 Primers

货号： 7859S 基本售价： 1374.0 元规格： 500ul

产品信息

概述
货号	7859S
描述	SimpleChIP® Human FKBP51 Intron 5 Primers contain a mix of forward and reverse PCR primers that are specific to the intron 5 region of the FK506 Binding Protein 51 (FKBP51) gene. These primers can be used to amplify DNA that has been isolated using chromatin immunoprecipitation (ChIP). Primers have been optimized for use in SYBR® Green quantitative real-time PCR and have been tested in conjunction with SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003 and ChIP-validated antibodies from Cell Signaling Technology®. FKBP51 is part of an immunophilin group of proteins that can bind immunosuppresive drugs such as FK506, which antagonize T lymphocyte proliferation through inhibition of calcineurin. FKBP51 plays roles in steroid hormone receptor function by interacting with HSP90 complexes containing androgens, glucocorticoids, and progestins.
反应种属	Human
应用	ChIP

性能
供应商	CST
存放说明	-20C
参考文献	Orlando, V. (2000) Trends Biochem Sci 25, 99-104. Kuo, M.H. and Allis, C.D. (1999) Methods 19, 425-33. Agalioti, T. et al. (2000) Cell 103, 667-78. Soutoglou, E. and Talianidis, I. (2002) Science 295, 1901-4. Mikkelsen, T.S. et al. (2007) Nature 448, 553-60. Lee, T.I. et al. (2006) Cell 125, 301-13. Weinmann, A.S. and Farnham, P.J. (2002) Methods 26, 37-47. Wells, J. and Farnham, P.J. (2002) Methods 26, 48-56.

参考图片

PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP® Human FKBP51 Intron 5 Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. The melt curve consists of 80 melt cycles, starting at 55°C with increments of 0.5°C per cycle. Each peak is formed from the degradation of a single PCR product.在使用SimpleChIP® Human FKBP51 Intron 5 Primers的real-time PCR反应中获得了PCR产物的溶解曲线。数据显示出两个使用20 ng总DNA为模板的重复PCR反应。溶解曲线是由80个溶解循环构成，从55°C开始并且每个循环增加0.5°C。每个峰都是单个PCR产物的解链。

SimpleChIP® Human FKBP51 Intron 5 Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R2) were calculated based on the corresponding threshold cycle (CT) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003在交联细胞中分离的DNA来测试SimpleChIP® Human FKBP51 Intron 5 Primers。通过real-time PCR检测系统和SYBR® Green reaction mix，取2% total input DNA连续稀释(20 ng, 4 ng, 0.8 ng, and 0.16 ng)，每个浓度做一个重复，进行Real-time PCR。PCR扩增效率（E）和相关系数（R2）的计算基于real-time PCR（95°C变性15秒，65°C退火/延伸60秒） 40个循环过程中每个稀释样品的相应的阈值周期（CT）。