| 货号 | 5148S |
| 描述 | SimpleChIP® Human TAP1 Promoter Primers contain a mix of forward and reverse PCR primers that are specific to a region of the human TAP1 promoter. These primers can be used to amplify DNA that has been isolated using chromatin immunoprecipitation (ChIP). Primers have been optimized for use in SYBR® Green quantitative real-time PCR and have been tested in conjunction with SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003 and ChIP-validated antibodies from Cell Signaling Technology®. The peptide transporter TAP1 gene is regulated by Stat proteins in response to interferon signaling. |
| 反应种属 | Human |
| 应用 | ChIP |
PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP® Human TAP1 Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. The melt curve consists of 80 melt cycles, starting at 55°C with increments of 0.5°C per cycle. Each peak is formed from the degradation of a single PCR product.从采用SimpleChIP™ Human TAP1Promoter Primers的实时定量PCR中得到PCR溶解曲线。数据是采用20ng总DNA两次PCR得到的。溶解曲线包括80个循环，起始温度位55度，每个循环增加0.5度。单一PCR产物的降解形成一个峰值。 | |
SimpleChIP® Human TAP1 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R2) were calculated based on the corresponding threshold cycle (CT) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 60°C anneal/extension for 60 sec). |
