SimpleChIP® Human Tyrosinase Promoter Primers【科瑞奥博】

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SimpleChIP® Human Tyrosinase Promoter Primers

货号： 12970S 基本售价： 1374.0 元规格： 500ul

产品信息

概述
货号	12970S
反应种属	Human
应用	ChIP
使用方法	1. Label the appropriate number of PCR tubes or PCR plates compatible with the model of real-time PCR machine to be used. PCR reactions should be performed in duplicate and should include a tube with no DNA to control for contamination, and a serial dilution of a 2% total input chromatin DNA (undiluted, 1:5, 1:25, 1:125), which is used to create a standard curve and determine amplification efficiency.

性能
供应商	CST
背景
存放说明	-20C
参考文献	Orlando, V. (2000) Trends Biochem Sci 25, 99-104. Kuo, M.H. and Allis, C.D. (1999) Methods 19, 425-33. Agalioti, T. et al. (2000) Cell 103, 667-78. Soutoglou, E. and Talianidis, I. (2002) Science 295, 1901-4. Mikkelsen, T.S. et al. (2007) Nature 448, 553-60. Lee, T.I. et al. (2006) Cell 125, 301-13. Weinmann, A.S. and Farnham, P.J. (2002) Methods 26, 37-47. Wells, J. and Farnham, P.J. (2002) Methods 26, 48-56.

参考图片

PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP^® Human Tyrosinase Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. The melt curve consists of 80 melt cycles, starting at 55°C with increments of 0.5°C per cycle. Each peak is formed from the degradation of a single PCR product.在使用SimpleChIP® Human Tyrosinase Promoter Primers的real-time PCR反应中获得了PCR产物的溶解曲线。数据显示出两个使用20 ng总DNA为模板的重复PCR反应。溶解曲线是由80个溶解循环构成，从55°C开始并且每个循环增加0.5°C。每个峰都是单个PCR产物的解链。

SimpleChIP^® Human Tyrosinase Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR^® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R²) were calculated based on the corresponding threshold cycle (C_T) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003在交联细胞中分离的DNA来测试SimpleChIP® Human Tyrosinase Promoter Primers。通过real-time PCR检测系统和SYBR® Green reaction mix，取2% total input DNA连续稀释(20 ng, 4 ng, 0.8 ng, and 0.16 ng)，每个浓度做一个重复，进行Real-time PCR。PCR扩增效率（E）和相关系数（R2）的计算基于real-time PCR（95°C变性15秒，65°C退火/延伸60秒） 40个循环过程中每个稀释样品的相应的阈值周期（CT）。