SimpleChIP® Rat CCRN4L Promoter Primers【科瑞奥博】

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SimpleChIP® Rat CCRN4L Promoter Primers

货号： 7983S 基本售价： 1374.0 元规格： 500ul

产品信息

概述
货号	7983S
描述	SimpleChIP® Rat CCRN4L Promoter Primers contain a mix of forward and reverse PCR primers that are specific to a region of the rat Carbon Catabolite Repression 4-Like (CCRN4L) promoter. These primers can be used to amplify DNA that has been isolated using chromatin immunoprecipitation (ChIP). Primers have been optimized for use in SYBR® Green quantitative real-time PCR and have been tested in conjunction with SimpleChIP® Enzymatic Chromatin IP Kits #9002 and #9003 and ChIP-validated antibodies from Cell Signaling Technology®. CCRN4L is a circadian rhythm deadenylase that acts downstream of Clock and Bmal1 to stabilize circadian-related mRNA transcripts.
反应种属	Rat
应用	ChIP

性能
供应商	CST
存放说明	-20C
参考文献	Orlando, V. (2000) Trends Biochem Sci 25, 99-104. Kuo, M.H. and Allis, C.D. (1999) Methods 19, 425-33. Agalioti, T. et al. (2000) Cell 103, 667-78. Soutoglou, E. and Talianidis, I. (2002) Science 295, 1901-4. Mikkelsen, T.S. et al. (2007) Nature 448, 553-60. Lee, T.I. et al. (2006) Cell 125, 301-13. Weinmann, A.S. and Farnham, P.J. (2002) Methods 26, 37-47. Wells, J. and Farnham, P.J. (2002) Methods 26, 48-56.

参考图片

PCR product melting curves were obtained for real-time PCR reactions performed using SimpleChIP® Rat CCRN4L Promoter Primers. Data is shown for both duplicate PCR reactions using 20 ng of total DNA. The melt curve consists of 80 melt cycles, starting at 55°C with increments of 0.5°C per cycle. Each peak is formed from the degradation of a single PCR product.在使用SimpleChIP® Rat CCRN4L Promoter Primers的real-time PCR反应中获得了PCR产物的溶解曲线。数据显示出两个使用20 ng总DNA为模板的重复PCR反应。溶解曲线是由80个溶解循环构成，从55°C开始并且每个循环增加0.5°C。每个峰都是单个PCR产物的解链。

SimpleChIP® Rat CCRN4L Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R2) were calculated based on the corresponding threshold cycle (CT) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003在交联细胞中分离的DNA来测试SimpleChIP® Rat CCRN4L Promoter Primers。通过real-time PCR检测系统和SYBR® Green reaction mix，取2% total input DNA连续稀释(20 ng, 4 ng, 0.8 ng, and 0.16 ng)，每个浓度做一个重复，进行Real-time PCR。PCR扩增效率（E）和相关系数（R2）的计算基于real-time PCR（95°C变性15秒，65°C退火/延伸60秒） 40个循环过程中每个稀释样品的相应的阈值周期（CT）。