| 货号 | 8104S |
| 供应商 | CST |
| 背景 | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly(ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). |
| 存放说明 | 4C |
| 参考文献 | Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. Nicholson, D.W. et al. (1995) Nature 376, 37-43. |
Immunohistochemical anaysis of paraffin-embedded Jurkat cells, untreated (left) or etoposide-treated (right), using Cleaved Caspase-3 (Asp175) Antibody #9661. 免疫组织化学分析石蜡包埋的Jurkat细胞，未经处理（左）或 etoposide 处理（右），使用抗体为Cleaved Caspase-3 (Asp175) Antibody #9661。 | |
Western blot analysis of extracts from Jurkat cells, untreated or treated with etoposide, using Cleaved Caspase-3 (Asp 175) (5A1) Rabbit mAb #9664 (upper) or Caspase-3 (8G10) Rabbit mAb #9665 (lower). This assay serves as a control for the efficacy of the etoposide treatment. Western blot 检测Jurkat细胞提取物，未处理 (-)或 etoposide 处理，使用抗体为Cleaved Caspase-3 (Asp 175) (5A1) Rabbit mAb兔单抗 #9664（上图）或Caspase-3 (8G10) Rabbit mAb兔单抗 #9665（下图）。本方法作为 etoposide 处理效率的对照。 | |
