| 货号 | 61021-AM |
| 克隆类别 | MABI 0333 |
| 同种亚型 | IgG1 |
| 反应种属 | Human, Mouse, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | ChIP/ChIP-Seq/Western Blot/Dot Blot |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP ChIP-Seq: 5 - 10 µg each WB: 0.5 - 2 µg/ml dilution ChIP-Seq validation was performed by Active Motifs Epigenetics Services and the complete data set is available in the UCSC Genome Browser by clicking here. Published Applications: ChIP See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human Histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a PBS only version of this antibody is also available. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3K36me3 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 15 ug of chromatin from a human medulloblastoma cell line and 4 µg of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify Histone H3K36me3 binding sites. The image shows binding across a region of chromosome 7. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here. | |
Histone H3K36me3 antibody (mAb) tested by Western blot HeLa nuclear extract (20 µg per lane) probed with Histone H3 trimethyl Lys36 antibody (2 µg/ml dilution). | |
Histone H3K36me3 antibody (mAb) tested by dot blot. Dot blot analysis was used to confirm the specificity of Histone H3K36me3 antibody for trimethyl Lys36 of histone H3. Recombinant methylated proteins corresponding to the immunogen and related sequences were spotted onto PVDF and probed with Histone H3K36me3 at 2 µg/ml. The amount of protein (picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified H3 Lys4. Lane 2: H3K4me1. Lane 3: H3K4me2. Lane 4: H3K4me3. Lane 5: unmodified H3 Lys9. Lane 6: H3K9me1. Lane 7: H3K9me2. Lane 8: H3K9me3. Lane 9: unmodified H3 Lys79. Lane 10: H3K79me1. Lane 11: H3K79me2. Lane 12: H3K79me3. Bottom panel - Lane 1: unmodified H3 Lys23. Lane 2: H3K23me1. Lane 3: H3K23me2. Lane 4: H3K23me3. Lane 5: unmodified H3 Lys27. Lane 6: H3K27me1. Lane 7: H3K27me2. Lane 8: H3K27me3. Lane 9: unmodified H3 Lys36. Lane 10: H3K36me1. Lane 11: H3K36me2. Lane 12: H3K36me3. |
