| 货号 | 39763 |
| 克隆类别 | MABI 0301 |
| 同种亚型 | IgG2b |
| 反应种属 | Human, Other (Wide Range) |
| 来源宿主 | Mouse |
| 应用 | ChIP/Western Blot/Immunofluorescence/Immunocytochemistry |
| 使用方法 | Validated Applications: ChIP: 5 - 10 µg per ChIP ICC/IF: 1 µg/ml dilution WB: 0.5 - 2 µg/ml dilution Published Applications: ChIP ICC/IF See references for more information. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Protein G Chromatography |
| 免疫原 | This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3. |
| 背景 | Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, a PBS only version of this antibody is also available. |
| 计算分子量 | 17 kDa |
| 参考文献 |
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Histone H3 antibody (mAb) tested by ChIP. ChIP performed on Active Motif Ready-to-ChIP HeLa cell chromatin (Catalog No. 53015) using Histone H3 antibody (mAb). PCR was performed using primers specific for the promoter region of the human GAPDH gene. Lane 1: ChIP using mouse IgG as a negative control. Lane 2: ChIP using 10 µg of Histone H3 antibody (mAb). Lane 3: Input DNA control. | |
Histone H3 antibody (mAb) tested by immunofluorescence. HeLa cells stained with Histone H3 antibody (mAb) at a dilution of 1 µg/ml. | |
Histone H3 antibody (mAb) tested by Western blot. HeLa nuclear extract (20 µg per lane) probed with Histone H3 antibody (mab) at a dilution of 2 µg/ml. Lane 1: Nuclear extract of untreated HeLa cells. Lane 2: Nuclear extract of HeLa cells treated with sodium butyrate. |
