| 货号 | 39000-AM |
| 别名 | CBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1 |
| 同种亚型 | IgG |
| 反应种属 | Human, Mouse |
| 来源宿主 | Rabbit |
| 应用 | Western Blot |
| 使用方法 | Validated Applications: WB: 1:500 - 1:1,000 dilution Published Applications: see references For optimal results, primary antibody incubations should be performed overnight at 4°C. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 免疫原 | This AML-1/Runx1 antibody was raised against a synthetic peptide corresponding to amino acid residues 231-245 of human AML-1/Runx1. |
| 背景 | AML-1(acute myeloid leukemia 1,Runx1,CBFA2,PEBP2αB) is a transcription factor (activator) for various hematopoietic-specific genes (for example, GM-CSF, CSF1R and TCRb). AML-1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML-1 is believed to be involved in the development of normal hematopoiesis and is commonly translocated in hematopoietic cancers. |
| 运输条件 | Blue Ice |
| 存放说明 | Antibodies in solution can be stored at -80°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage. |
| 存储溶液 | PBS containing 0.2% gelatin, 0.01% thimersol and 0.1% sodium azide. Thimersol and sodium azide are highly toxic. |
| 计算分子量 | 53 kDa |
| 参考文献 |
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AML-1/Runx1 antibody tested by Western blot. Detection of AML-1/Runx1 by Western blot analysis. Positive detection of AML-1/Runx1 in nuclear extracts derived from Jurkat cells at 1:1,000 (lane 1), 1:500 (lane 2), and 1:250 (lane 3) dilutions. |
