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货号： bs-13344R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-13344R
- 英文名称
- GGA2
- 中文名称
- γ-衔接蛋白相关蛋白2抗体
- 别 名
- 1200007E24Rik; ADP ribosylation factor binding protein 2; ADP ribosylation factor binding protein GGA 2; ADP ribosylation factor binding protein GGA2; ADP-ribosylation factor-binding protein GGA2; ARF-binding protein 2; FLJ20966; Gamma adaptin related protein 2; gamma ear-containing; Gamma-adaptin-related protein 2; GGA 2; Gga2; GGA2_HUMAN; Golgi associated gamma adaptin ear containing ARF binding protein 2; Golgi localized gamma ear containing ARF binding protein 2; Golgi-localized; KIAA1080; Vear; VHS domain and ear domain containing protein; VHS domain and ear domain of gamma adaptin; VHS domain and ear domain of gamma-adaptin.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 心血管 细胞生物 信号转导 转运蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 67kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human GGA2:161-260/613
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
A family of proteins, the GGAs (Golgi-localized, g-adaptin ear-containing, ARF-binding proteins) sequences that showed significant homology to the carboxy-terminal ear domain of g-adaptin. Members of the GGA family (GGA1,GGA2 (also known as VEAR or VHS domain and ear domain of g-adaptin) and GGA3) are ubiquitous coat proteins that facilitate the trafficking of proteins between the trans-Golgi network and the lysosome. However, unlike g-adaptin, the GGAs are not associated with clathrin-coated vesicles or with any of the components of the AP-1 complex. GGA1 and GGA2 are also not associated with each other, although they colocalize on perinuclear membranes. GGA2 shares 45% amino acid sequence identity with GGA1 and 35% with GGA3. In addition to being involved in heterotypic vesicle/suborganelle interactions associated with the Golgi complex, GGA2 may have a tissue-specific function and is highly expressed in kidney, muscle and heart. Furthermore, the VHS domain of GGA2 binds to the acidic cluster-di-leucine motif in the cytoplasmic tail of the cation-independent mannose 6-phosphate receptor (CI-MPR) and this is important for lysosomal enzyme targeting.
Function:
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
Subunit:
Monomer. Interacts with NECAP1, TSG101, UBC and AFTPH/aftiphilin. Interacts with CNST (By similarity). Interacts with GGA1 and GGA3. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the type-I membrane proteins SORT1, SORL1, LRP3, M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. Binds the accessory proteins CCDC91, P200, SYNRG, EPN4 and NECAP2.
Subcellular Location:
Golgi apparatus; trans-Golgi network membrane. Endosome membrane.
Tissue Specificity:
Ubiquitously expressed.
Post-translational modifications:
Ubiquitinated.
Similarity:
Belongs to the GGA protein family.
Contains 1 GAE domain.
Contains 1 GAT domain.
Contains 1 VHS domain.
SWISS:
Q9UJY4
Gene ID:
23062
Database links:Entrez Gene: 23062 Human
Entrez Gene: 74105 Mouse
Entrez Gene: 293455 Rat
Omim: 606005 Human
SwissProt: Q9UJY4 Human
SwissProt: Q6P5E6 Mouse
Unigene: 460336 Human
Unigene: 29619 Mouse
Unigene: 18248 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GGA2 Polyclonal Antibody, Unconjugated(bs-13344R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell:Rat brian; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GGA2, Unconjugated(bs-13344R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GGA2 Polyclonal Antibody, Unconjugated(bs-13344R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining