Glucose Uptake Colorimetric Assay Kit
货号：
K676-100 基本售价：
11180.0 元 规格：
100assays
产品信息
概述| 货号 | K676-100 |
| 描述 | Glucose uptake is an important biological process for studying cell signaling and glucose metabolism. Among many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells. In BioVision’s glucose uptake colorimetric assay kit, the 2-DG6P is oxidized to generate NADPH, which can be determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (412 nm)• Species reactivity- Mammalian • Applications- This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/well. |
| 样本类型以及上样量 | Living cells (suspension and adherent) |
| 产品特色 | • Simple procedure• Fast and convenient• The assay is easy, non-radioactive, and highly sensitive |
| 试剂盒组分 | • Extraction Buffer• Neutralization Buffer• 2-Deoxyglucose (2-DG, 10 mM)• Assay Buffer• Enzyme Mix (lyophilized)• Recycling Mix (lyophilized)• 2-DG6P Standard (lyophilized)• Glutathione Reductase (lyophilized)• Substrate-DTNB (lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
