AHCY Inhibitor Screening Kit (Fluorometric)
货号：
K326-100 基本售价：
10220.0 元 规格：
100assays
产品信息
概述| 货号 | K326-100 |
| 描述 | Adenosyl homocysteinase (AHCY) (EC 3.3.1.1) or S-adenosyl homocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. Inhibition of AHCY results in the accumulation of SAH, a product inhibitor of S-adenosyl methionine (SAM)-dependent methyltransferases. AHCY is an important target as an antiviral and anticancer drug. Several characterized SAH inhibitors inhibit some DNA viruses (e.g. Pox viruses) and some negative stranded RNA viruses (e.g.: Marburg virus, Ebola virus & rabies). In BioVision’s AHCY Inhibitor Screening kit the homocysteine generated from the breakdown of SAH is measured using a Thiol Detecting Reagent resulting in enhanced fluorescence which is measured at Ex/Em = 392/482 nm. The AHCY reaction is reversible and hence Adenosine Deaminase is included in the reaction to ensure that the reaction proceeds towards hydrolysis of SAH. In the presence of AHCY inhibitor, there is a decrease in fluorescence of the Thiol Detecting Reagent. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 392/482 nm)• Applications - Screening/characterizing/studying AHCY inhibitors |
| 产品特色 | • Simple and reliable test to screen AHCY inhibitors• High-throughput suitable• Includes Inhibitor Control (3-Deazaneplanocin A) |
| 试剂盒组分 | • AHCY Assay Buffer• AHCY Reconstitution Buffer• AHCY Enzyme• Adenosine Deaminase • 3-Deazaneplanocin A (10 µM) (in DMSO)• AHCY Substrate (in DMSO)• Thiol Detecting Reagent (in DMSO) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
