Ghrelin (human, mouse, rat) EIA Kit
货号：
K4790-100 基本售价：
9033.0 元 规格：
100assays
产品信息
概述| 货号 | K4790-100 |
| 描述 | BioVision’s Ghrelin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Ghrelin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Ghrelin antibody, both biotinylated Ghrelin peptide and peptide standard or targeted peptide in samples interacts competitively with the Ghrelin antibody. Uncompeted (bound) biotinylated Ghrelin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Ghrelin peptide in the standard or samples. This is due to the competitive binding of Ghrelin antibody between biotinylated Ghrelin peptide and peptides in standard or samples. A standard curve of known concentration of Ghrelin peptide can be established and the concentration of Ghrelin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Ghrelin is 161 pg/ml or 12.46 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<15%. This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Nesfatin, Angiotensin II, NPY and APC. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity - human, mouse, rat• Applications - Quantitative protein detection, establishing normal range, validation of antibody array results |
| 样本类型以及上样量 | • Serum & plasma• Cell culture media or other sample types |
| 产品特色 | • Easy, convenient and time-saving method to assay Ghrelin. • The minimum detectable concentration of Ghrelin is less than 161 pg/ml . • This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Nesfatin, Angiotensin II, NPY and APC. |
| 试剂盒组分 | • Ghrelin Microplate (Item A) coated with secondary antibody, 96 wells• Wash Buffer Concentrate (20x) (Item B)• Lyophilized Standard Ghrelin Peptide (Item C) • Lyophilized anti-Ghrelin Polyclonal antibody (Item N)• 5 X Assay Diluent B (Item E) • Lyophilized Biotinylated Ghrelin peptide (Item F)• HRP-Streptavidin Concentrate (Item G), 100x concentrated• Lyophilized Positive Control (Item M) • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
