Nesfatin (human/mouse/rat) EIA Kit
货号：
K4758-100 基本售价：
9033.0 元 规格：
100assays
产品信息
概述| 货号 | K4758-100 |
| 描述 | BioVision’s Nesfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Nesfatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Nesfatin antibody, both biotinylated Nesfatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Nesfatin antibody. Uncompeted (bound) biotinylated Nesfatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Nesfatin peptide in the standard or samples. This is due to the competitive binding to Nesfatin antibody between biotinylated Nesfatin peptide and peptides in standard or samples. A standard curve of known concentration of Nesfatin peptide can be established and the concentration of Nesfatin peptide in the samples can be calculated accordingly. EIA-NES-1 detects 420aa Nesfatin protein. No other active isoforms have been reported. The minimum detectable concentration of Nesfatin is 147 pg/ml or 3.09 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Species reactivity- human, mouse, rat • Applications- Quantitative protein detection, establishing normal range, validation of antibody array results. |
| 样本类型以及上样量 | • Serum & plasma • Cell culture media etc. |
| 产品特色 | • Easy, convenient and time-saving method to assay Nesfatin. • The minimum detectable concentration of Nesfatin is typically less than 147 pg/ml. • This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Angiotensin II, NPY and APC. |
| 试剂盒组分 | • Nesfatin Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondary antibody. • Wash Buffer Concentrate (20x) (Item B): 25 ml. • Standard Nesfatin peptide (Item C): 2 vials, 10 ul/vial. • Anti- Nesfatin polyclonal antibody (Item N): 2 vials, 5 ul/vial. • Assay Diluent A (Item D): 30 ml, contains 0.09% sodium azide as preservative. Diluent for standards and serum or plasma samples. • Assay Diluent B (Item E): 15 ml, 5X concentrated buffer. Diluent for standards and cell culture media or other sample types. • Biotinylated Nesfatin peptide (Item F): 2 vials, 20 ul/vial. • HRP-Streptavidin concentrate (Item G): 600 µl 200x concentrated HRP-conjugated Streptavidin. • Positive control (Item M): 1 vial, 100 ul. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3, 3’, 5, 5’- tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
