C-Peptide (human/mouse/rat) EIA Kit
货号：
K4757-100 基本售价：
9033.0 元 规格：
100assays
产品信息
概述| 货号 | K4757-100 |
| 描述 | BioVision’s C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- C-Peptide antibody, both biotinylated C-Peptide peptide and peptide standard or targeted peptide in samples interacts competitively with the C-Peptide antibody. Uncompeted (bound) biotinylated C-Peptide peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of C-Peptide peptide in the standard or samples. This is due to the competitive binding to C-Peptide antibody between biotinylated C-Peptide peptide and peptides in standard or samples. A standard curve of known concentration of C-Peptide peptide can be established and the concentration of C-Peptide peptide in the samples can be calculated accordingly. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain. The minimum detectable concentration of C-Peptide is 772 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Species reactivity- human, mouse, rat • Applications- Quantitative protein detection, establishing normal range, validation of antibody array results. |
| 样本类型以及上样量 | • Serum & plasma • Cell culture media etc. |
| 产品特色 | • Easy, convenient and time-saving method to assay C-Peptide. • The minimum detectable concentration of C-Peptide is typically less than 772 pg/ml. • This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC. |
| 试剂盒组分 | • C-Peptide Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondary antibody. • Wash Buffer Concentrate (20x) (Item B): 25 ml. • Standard C-Peptide peptide (Item C): 2 vials, 10 ul/vial. • Anti- C-Peptide polyclonal antibody (Item N): 2 vials, 5 ul/vial. • Assay Diluent A (Item D): 30 ml, contains 0.09% sodium azide as preservative. Diluent for standards and serum or plasma samples. • Assay Diluent B (Item E): 15 ml, 5X concentrated buffer. Diluent for standards and cell culture media or other sample types. • Biotinylated C-Peptide peptide (Item F): 2 vials, 20 ul/vial. • HRP-Streptavidin concentrate (Item G): 600 µl 200x concentrated HRP-conjugated Streptavidin. • Positive control (Item M): 1 vial, 100 ul. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3, 3’, 5, 5’- tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
