Glucagon (human/mouse/rat) EIA Kit
货号：
K4756-100 基本售价：
9033.0 元 规格：
100assays
产品信息
概述| 货号 | K4756-100 |
| 描述 | BioVision’s Glucagon Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Glucagon antibody, both biotinylated Glucagon peptide and peptide standard or targeted peptide in samples interacts competitively with the Glucagon antibody. Uncompeted (bound) biotinylated Glucagon peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Glucagon peptide in the standard or samples. This is due to the competitive binding to Glucagon antibody between biotinylated Glucagon peptide and peptides in standard or samples. A standard curve of known concentration of Glucagon peptide can be established and the concentration of Glucagon peptide in the samples can be calculated accordingly. The minimum detectable concentration of Glucagon is 0.97 pg/ml. The detection range for the kit is 1-1,000 pg/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Species reactivity- human, mouse, rat • Applications- Quantitative protein detection, establishing normal range, validation of antibody array results. |
| 样本类型以及上样量 | • Serum & plasma • Cell culture media etc. |
| 产品特色 | • Easy, convenient and time-saving method to assay for Glucagon. • The minimum detectable concentration of Glucagon is typically less than 0.97 pg/ml. • This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC. |
| 试剂盒组分 | • Glucagon Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondary antibody. • Wash Buffer Concentrate (20x) (Item B): 25 ml. • Lyophilized standard Glucagon peptide (Item C): 2 vials. • Lyophilized anti-Glucagon polyclonal antibody (Item N): 2 vials • 5X Assay Diluent B (Item E), Diluent for both standards and samples including serum or plasma, cell culture media or other sample types.• Lyophilized biotinylated Glucagon peptide (Item F): 2 vials. • HRP-Streptavidin concentrate (Item G): 600 µl 200x concentrated HRP-conjugated Streptavidin. • Lyophilized positive control (Item M): 1 vial. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3, 3’, 5, 5’- tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
