HDAC1 Immunoprecipitation (IP) & Activity Assay Kit
货号：
K342-25 基本售价：
9033.0 元 规格：
25assays
产品信息
概述| 货号 | K342-25 |
| 描述 | Histone deacetylases (HDACs) play a central role in controlling cell cycle regulation, cell differentiation, and tissue development. HDAC1 is ubiquitously expressed and plays a role in cellular senescence, cardiomyocyte differentiation and hypertrophy, aging of the liver, myelination, and adult neurogenesis to name a few functions. The HDAC1 IP & Activity Assay Kit provides an antibody-based method to specifically immunoprecipitate the HDAC1 complex from cells & tissues and to measure HDAC1 activity fluorometrically. HDAC1 is immunoprecipitated from cell, nuclear or tissue extract(s) using HDAC1 antibody followed by capturing the complex on protein-A/G beads. The immunoprecipitated complex is incubated with the HDAC substrate. Only the deacetylated substrate is cleaved by the Developer to produce a fluorophore, which can be easily analyzed using a fluorescence plate reader. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em=380/500 nm) • Applications: - Immunoprecipitation of HDAC1 complex from cell, nuclear, or tissue extract(s) - Measurement of HDAC1 activity of immunoprecipitated complex/purified enzyme - Screening for activators or inhib |
| 样本类型以及上样量 | • Human, Mouse or Rat • Cell Lysate and Nuclear Extract • Tissue Extract |
| 产品特色 | • Simple, fast and convenient. • Only kit on the market for both IP as well as measuring activity of HDAC1 |
| 试剂盒组分 | • HDAC Assay Buffer • Cell Lysis Buffer • HDAC Substrate • Developer • AFC (7-amino-4-trifluoromethyl coumarin) Standard (1 mM) • Rabbit HDAC1 Antibody • Rabbit IgG (Control Antibody) • Protein-A/G Sepharose Beads (50% slurry in 20% ethanol/H2O) • Positive Control (Jurkat Cell Lysate) Lyophilized |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
