Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit
货号：
K807-100 基本售价：
8851.5 元 规格：
100assays
产品信息
概述| 货号 | K807-100 |
| 描述 | Adenosylhomocysteinase (AHCY) (EC 3.3.1.1) or S-adenosylhomocysteine hydrolase (SAHH); is an enzyme that catalyzes the reversible hydrolysis of S-Adenosyl Homocysteine (SAH) to adenosine and homocysteine. AHCY regulates the intracellular SAH concentration which in turn regulates S-adenosyl methionine (SAM)-dependent methyltransferases. Down-regulation of AHCY has been associated with certain forms of cancer and Huntington’s disease, while in Wilson’s disease; the enzyme is inhibited by the accumulated copper. Mutations in the AHCY gene cause SAHH deficiency disease. BioVision’s AHCY Activity Assay kit can kinetically measure AHCY activity by detecting adenosine generation resulting from the hydrolysis of SAH. Adenosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the OxiRed™ Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification (L.O.Q) is 1 µU recombinant human AHCY. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 535/587 nm)• Applications - Detection of AHCY activity |
| 样本类型以及上样量 | • Purified recombinant protein• Cell and tissue lysate |
| 产品特色 | • Rapid, simple & convenient• The assay can detect AHCY activity as low as 1 µU recombinant human AHCY. |
| 试剂盒组分 | • AHCY Assay Buffer• Homogenization Buffer• OxiRed™ Probe• SAH Substrate• Enzyme Mix I • Enzyme Mix II • Adenosine Standard (10 mM)• AHCY Enzyme (Positive Control) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
