Enolase Activity Colorimetric/Fluorometric Assay Kit
货号：
K691-100 基本售价：
7883.5 元 规格：
100assays
产品信息
概述| 货号 | K691-100 |
| 描述 | Enolase (EC 4.2.1.11), also called 2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) & also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, Enolase consists of three subunits: α, β and γ that combine into 5 isozymes, 3 of which are commonly found in human tissues. This enzyme exists in all organisms, which can undergo glycolysis. Besides its role in glycolysis, enolase has other functions too. Recent studies have shown that increased activity of enolase is associated with tumor invasion and metastasis; therefore, precise measurement of enolase activity may be of great interest for tumor diagnosis and prognosis. In BioVision’s Enolase Activity Assay Kit, enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm)• Applications - Measurement of enolase activity in various tissues/cells- Analysis of glycolytic metabolism in different tissues/cells- Mechanistic studies of various cancers- Screen |
| 样本类型以及上样量 | • Animal tissues: Liver, muscle, heart, kidney etc.• Cell culture: adherent or suspension cells |
| 产品特色 | • Rapid and easy-to-use.• Highly-sensitive• This assay kit can measure less than 0.04 mU of enolase activity in a variety of samples. |
| 试剂盒组分 | • Enolase Assay Buffer• Enolase Substrate Mix (Lyophilized)• Enolase Converter (Lyophilized)• Enolase Developer (Lyophilized)• Enolase Positive Control (Lyophilized)• H2O2 Standard (0.88 M) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
