Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)
货号：
K767-100 基本售价：
7762.5 元 规格：
100assays
产品信息
概述| 货号 | K767-100 |
| 描述 | Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T- cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems. In BioVision’s Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 535/587 nm)• Applications - Detection of Purine Nucleoside Phosphorylase activity in variety of samples |
| 样本类型以及上样量 | • Purified recombinant protein• Cell and tissue lysate |
| 产品特色 | • Rapid, simple & convenient• Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase |
| 试剂盒组分 | • PNP Assay Buffer (10x)• Enzyme Mix • Inosine Substrate• PNP Probe (in dry DMSO)• Hypoxanthine Standard (10 mM)• PNP Positive Control |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
