Succinyl-CoA Synthetase Activity Colorimetric Assay Kit
货号：
K597-100 基本售价：
7762.5 元 规格：
100assays
产品信息
概述| 货号 | K597-100 |
| 描述 | Succinyl-CoA Synthetase (SCS, also called Succinyl-CoA ligase, Succinate Thiokinase) (EC 6.2.1.5) is a critical enzyme in the citric acid cycle and an important metabolic intermediate for porphyrin, heme and ketone body biosynthesis. It is located in the mitochondrial matrix and is a heterodimer composed of one α and one β subunit. In humans, Succinyl-CoA Synthetase deficiency causes the build-up of lactic acid leading to lactic acidosis, which can be fatal in infants. Measurement and analysis of SCS activity is useful for both mechanistic studies as well as for diagnostic purposes. In BioVision’s Succinyl-CoA Synthetase Activity Assay, SCS converts succinate into succinyl-CoA in the presence of ATP and CoA. Succinyl-CoA reacts with the Developer to form a colored product with strong absorbance at 450 nm. This assay kit is simple, sensitive, and high-throughput adaptable. It can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (OD 450 nm).• Applications - Measurement of Succinyl-CoA Synthetase activity in various tissues/cells - Analysis of cell signaling pathway |
| 样本类型以及上样量 | • Animal tissues: heart, liver, muscle, etc.• Purified mitochondria• Cell culture: adherent or suspension cells |
| 产品特色 | • Simple & Rapid Protocol• High-Throughput adaptable• This kit can detect less than 0.1 mU of Succinyl-CoA Synthetase activity in a variety of samples. |
| 试剂盒组分 | • SCS Assay Buffer• SCS Substrate Mix (Lyophilized)• SCS Enzyme Mix (Lyophilized) • SCS Developer (Lyophilized)• NADH Standard (Lyophilized)• SCS Positive Control (Lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
