PicoProbeTM Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit
货号：
K673-100 基本售价：
7641.5 元 规格：
100assays
产品信息
概述| 货号 | K673-100 |
| 描述 | Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes. Biovision’s PicoProbeTM Dihydroxyacetone Phosphate Assay kit is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, TPI converts DHAP to GAP that undergoes a series of reaction and reduces PicoProbe™ to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of Dihydroxyacetone Phosphate. This simple, sensitive, and easy to use assay kit can detect Dihydroxyacetone Phosphate as low as 0.5 µM in a variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 535/587 nm)• Applications - Measurement of Dhydroxyacetone Phosphate in various tissues/cells• Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis&bu |
| 样本类型以及上样量 | • Animal tissues: e.g., liver, muscle etc.• Cell culture: adherent or suspension cells• Biological fluids: serum, plasma etc. |
| 产品特色 | • Simple, Sensitive & Rapid protocol• Highly-Sensitive: Detects as low as 0.5 µM DHAP |
| 试剂盒组分 | • DHAP Assay Buffer• PicoProbe™ (in DMSO)• DHAP Enzyme Mix • DHAP Developer • DHAP Standard |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
