Gluconokinase (GntK) Activity Assay Kit (Colorimetric)
货号：
K319-100 基本售价：
7520.5 元 规格：
100assays
产品信息
概述| 货号 | K319-100 |
| 描述 | Gluconokinase (ATP:D-gluconate 6-phosphotransferase or Gluconate Kinase; EC:2.7.1.12) is a key enzyme for Gluconate degradation pathway. In E. coli and yeast, Gluconokinase can convert gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. Through Hexose Monophosphate Shunt (HMS) pathway, 6-Phosphate-D-Gluconate generates ribose-6-phosphate, which is critical for nucleotides and nucleic acid synthesis. Little is known of the mechanism of gluconate metabolism in humans despite its widespread use in medicine and consumer products. BioVision’s Gluconokinase Assay kit provides a quick and easy way for monitoring Gluconokinase activity in a variety of samples. In this kit, Gluconokinase converts Gluconate into 6-Phosphate-D-Gluconate in an ATP dependent manner. 6-Phosphate-D-Gluconate and ADP in turn undergoe a series of reactions to form an intermediate, which reacts with the probe to form a colored product with strong absorbance (OD 450 nm). The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1mU. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Applications - Measurement of Gluconokinase activity in various samples- Mechanistic study of Pentose Phosphate Pathway |
| 样本类型以及上样量 | • Prokaryote such as: E.coli • Animal tissues such as liver, kidney, etc. • Adherent or suspension cells. |
| 产品特色 | • Simple, rapid & convenient• The assay can detect gluconokinase activity less than 0.1 mU in a variety of samples |
| 试剂盒组分 | • Gluconokinase Assay Buffer• Gluconokinase Substrate • ATP • Gluconokinase Converting Enzyme • Gluconokinase Developer • Gluconokinase Probe• NADH Standard • Gluconokinase Positive Control |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
