Myeloperoxidase (MPO) Inhibitor Screening Kit (Fluorometric)
货号：
K746-200 基本售价：
7399.5 元 规格：
200assays
产品信息
概述| 货号 | K746-200 |
| 描述 | Myeloperoxidase (MPO: EC 1.11.2.2) is a peroxidase abundantly expressed in neutrophil granulocytes. It catalyzes the hydrogen peroxide dependent oxidation of halide ions to generate hypochlorite (HClO), the reaction by which MPO exhibits cytotoxic activity against tumor cells and microorganisms. MPO also oxidizes various substances such as phenol and anilines. MPO undergoes chlorination or peroxidation reaction depending upon the relative concentrations of chloride and the reducing substrates. Recent studies suggest that increased levels of MPO are associated with an increased risk for cardiovascular disease and myocardial infarction, thus development of novel and specific inhibitors of MPO is critical for therapeutic purposes. BioVision’s MPO Inhibitor Screening Kit provides screening assays for both MPO chlorination and peroxidation activities. In the chlorination assay (specific for MPO), MPO converts hydrogen peroxide and sodium chloride to sodium hypochlorite, which reacts with Chlorination substrate to give an intensely fluorescent product (Ex/Em = 480/520 nm). In the Peroxidation Assay, MPO oxidizes peroxidation substrate to generate fluorescence (Ex/Em = 535/587 nm). The fluorescence generated is directly proportional to any peroxidase activity present. In the presence of MPO inhibitor, reactions are impeded, thus decreasing the rate and/or extent of generation of MPO-dependent fluorescence. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of MPO, and identifying whether one or both activities are inhibited. MPO Inhibitor Control is included to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 20 min. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 535/587 nm)• Applications - Screening/characterizing/studying potential inhibitors of MPO |
| 产品特色 | • Rapid and simple • Reliable test for screening potential inhibitors of MPO• Includes Inhibitor Control [4-Aminobenzonic Hydrazide] |
| 试剂盒组分 | • MPO Assay Buffer• MPO Chlorination Substrate (in DMSO)• MPO Peroxidation Substrate (in DMSO)• MPO Enzyme• Inhibitor Control (4-Aminobenzonic Hydrazide, in DMSO)• Hydrogen Peroxide |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
