Akt Activity Assay Kit, KinaseSTAR
货号：
K435-40 基本售价：
6915.5 元 规格：
40assays
产品信息
概述| 货号 | K435-40 |
| 描述 | Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate. Phosphorylation of the GSK-3α can be analyzed by Western blot analysis using the phospho-GSK-3α specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions • Sample type- Cell and Tissue lysates • Species reactivity- Mammalian • Applications- The Akt Activity Assay Kit utilizes an Akt-specific an |
| 产品特色 | • Simple procedure • The kit is suitable for both research and drug discovery • The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected. |
| 试剂盒组分 | • Kinase Extraction Buffer • Akt Specific Antibody • Protein A Sepharose • GSK-3α Protein/ATP Mixture • Kinase Assay Buffer • Phospho-GSK-3α Specific Antibody |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
