NFkB Secreted Alkaline Phosphatase Reporter Assay Kit
货号：
NBP2-25286 基本售价：
6821.2 元 规格：
1 Kit
产品信息
概述| 货号 | NBP2-25286 |
| 反应种属 | HuSpecies Glossary |
| 应用 | ELISA |
| 目标/特异性 | Plasmids included in the kit is for one 96 well plate transfection. Other reagents included in the kit are for four 96 well plate SEAP Assays |
性能| 供应商 | Novus |
| 免疫原 | NFkB Secreted Alkaline Phosphatase Assay Kit for fast and sensitive detection of NF-KB activity. |
| 背景 | Placental alkaline phosphatase (PLAP) is one of the most stable isoenzymes, only existing in the placenta of higher primates. These characteristics make placental alkaline phosphatase suitable to use as a reporter gene for the analysis of promoter activity and gene expression in cell culture and animal serum. The natural form of PLAP is membrane anchored. The recombinant form of placental alkaline phosphatase (secreted alkaline phosphatase, SEAP) is used for reporter gene function. SEAP is designed by inserting a translational terminator after amino acid 489 (Berger, et al., Gene 66 (1): 10 (1988). This mutation converts the membrane-bound PLAP protein into the secreted protein SEAP. As a major transcription factor NF-KB plays a key role in regulating genes responsible for the innate and adaptive immune responses. In unstimulated cells, the NF-KB dimers are held in cytoplasm by IKBs that masks the nuclear localization signals of NF-KB. Upon cell stimulation, which leads to IKB degradation, NF-KB quickly translocates to the nucleus and activates various genes that have DNA-binding sites for NF-KB. Our NF-KB SEAPORTER Assay Kit is designed to measure NF-KB activation using SEAP protein secreted to the culture media as a read-out. |
| 存放说明 | Store at -20C. Avoid freeze-thaw cycles. |
