D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric)
货号：
K213-100 基本售价：
6673.5 元 规格：
100assays
产品信息
概述| 货号 | K213-100 |
| 描述 | In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and α-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of α-ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies. BioVision’s D-2-Hydroxyglutarate Assay Kit provides a convenient method to detect D2HG in biological samples. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 10 µM in various samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Detection method- Absorbance (OD= 450 nm). • Species reactivity - Mammalian• Applications - Measurement of D2HG level in various cell/tissues/biological fluids. |
| 样本类型以及上样量 | • Adherent or suspension Cells: e.g. 3T3, HepG2, Jurkat cells.• Tissues: e.g. Rat Liver, Rat Kidney, etc.• Biological Samples: Urine. |
| 产品特色 | • Simple, fast, HTP adaptable and convenient assay• Can measure D2HG levels as low as 10 µM in various samples. |
| 试剂盒组分 | • D2HG Assay Buffer• D2HG Enzyme• D2HG Substrate Mix• D2HG Standard |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
