| 货号 | 12581S |
| 反应种属 | All |
| 应用 | FUNC |
| 目标/特异性 | The Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit detects sample G6PD activity. The presence of NADH and NADPH may interfere with the assay. |
| 供应商 | CST |
| 背景 | Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first, and rate-limiting, step of the pentose phosphate pathway (1). The NADPH generated from this reaction is essential to protect cells from oxidative stress (1). Research studies have shown that p53 interacts with G6PD and inhibits its activity, therefore suppressing glucose consumption through the pentose phosphate pathway (2). In cancer cells with p53 mutations, the increased glucose consumption is directed towards increased biosynthesis, which is critical for cancer cell proliferation (2). |
| 存放说明 | -20C |
| 参考文献 | Au, S.W. et al. (2000) Structure 8, 293-303. Jiang, P. et al. (2011) Nat Cell Biol 13, 310-6. |
Figure 3. The relationship between the protein concentration of lysates from untreated and G6PD inhibitor DHEA (0.5 mM) treated Jurkat cells and relative fluorescence (RFU) is shown. The G6PD inhibitor DHEA can effectively inhibit this chain reaction as shown in this figure. | |
Figure 2. Each assay component is individually omitted from the assay system and the resultant RFU is compared to that of a control test that contains all of the assay components. | |
Figure 1. Schematic diagram of glucose-6-phosphate dehydrogenase (G6PD) assay. Glucose-6-phosphate (G6P) is oxidized by G6PD in the presence of NADP, which generates 6-phosphogluconolactone and NADPH. The generated NADPH is then amplified by the diaphorase-cycling system to produce highly fluorescent resorufin molecules. |
