Butyrylcholinesterase Activity Kit (Colorimetric)
货号：
K516-100 基本售价：
6552.5 元 规格：
100assays
产品信息
概述| 货号 | K516-100 |
| 描述 | Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as plasma cholinesterase or pseudocholinesterase, is a serine hydrolase present in almost all mammalian tissues with the highest levels detected in plasma and liver. BChE hydrolyzes choline esters, as well as other esters and acts as an endogenous scavenger for anticholinesterase agents. BChE in plasma serves as the first line of defense against toxic compounds reaching the bloodstream that might inhibit acetylcholinesterase activity, which is essential in the nervous system. In clinical toxicology and clinical chemistry, determination of BChE activity in plasma is the most commonly used and preferred method to diagnose patients showing symptoms of intoxication. A recent study using genome-wide analysis suggested that BChE is a marker of obesity, insulin resistance and metabolic syndrome, hyperlipidemia, coronary artery disease and hypertension. Serum BChE has been proposed as a marker of low-grade systemic inflammation and a marker of cardiovascular risk factor being even capable to predict mortality. Biovision’s Butyrylcholinesterase Activity Kit is based on the ability of BChE to hydrolyze substrate and produce thiocholine. Thiocholine reacts with 5, 5’-dithiobis (2-nitrobenzoic acid) (DTNB) and generates a yellow chromophore that can be quantified at 412 nm. The assay is simple, sensitive and can detect as low as 0.2 U/ml in variety of samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (412 nm)• Species reactivity - Mammalian• Applications - Measurement of Butyrylcholinesterase activity in various biological samples and purified/crude enzyme preparations |
| 样本类型以及上样量 | • Biological Fluids such as plasma• Animal tissues such as rat liver and lung• Purified enzyme |
| 产品特色 | • Simple, rapid & convenient assay to measure Butyrylcholinesterase• High-throughput adaptable• Includes Positive Control (Butyrylcholinesterase) |
| 试剂盒组分 | • BChE Assay Buffer• BChE Substrate (in DMSO)• Butyrylcholinesterase• DNTB• TNB Standard |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
