| 货号 | K675-100 |
| 描述 | Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer. BioVision’s Sulfatase Activity Assay Kit provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at 515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
| 供应商 | Biovision |
| 样本类型以及上样量 | • Animal tissues such as liver, endometrium, and breast cancer • Adherent or suspension cells • Purified enzyme |
| 试剂盒组分 | • Sulfatase Assay Buffer • Sulfatase Substrate • Stop/Developing Solution • Sulfatase • 4-Nitrocatechol Standard (0.5 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |