N′-Nicotinamide Methyltransferase (NNMT) Inhibitor Screening Assay Kit
货号：
K822-100 基本售价：
6189.5 元 规格：
100assays
产品信息
概述| 货号 | K822-100 |
| 描述 | N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy. Biovision’s NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using a Thiol Detecting Probe generating enhanced fluorescence signal that can be measured at Ex/Em = 392/482 nm. In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT. |
| 同种亚型 | Rabbit IgG1 |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 392/482 nm)• Species reactivity- Mammalian• Application - Screening/studying/characterizing potential inhibitors of NNMT |
| 产品特色 | • Rapid assay and simple protocol• Reliable test for screening potential inhibitors of NNMT• Includes Inhibitor Control, 1-Methylnicotinamide (MNA) |
| 试剂盒组分 | • NNMT Assay Buffer• NNMT Enzyme • S-Adenosylmethionine (SAM) • Nicotinamide • Enzyme-I• Enzyme-II• 1-Methylnicotinamide (MNA) (150 mM)• Thiol Detecting Probe (DMSO)• SAM Reconstitution Buffer |
| 运输条件 | Dry Ice |
| 存放说明 | -80℃ |
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