β-Glucuronidase Activity Assay Kit (Fluorometric)
货号：
K514-100 基本售价：
6189.5 元 规格：
100assays
产品信息
概述| 货号 | K514-100 |
| 描述 | β-Glucuronidases are hydrolytic enzymes responsible for the breakdown of carbohydrates. Specifically, β-Glucuronidases cleave the terminal β-D-glucuronic acid residue from the non-reducing terminus of a mucopolysaccharide chain. In humans, these enzymes are found in the lysosome of many tissue types. Loss of β-Glucuronidase activity results in a metabolic disease known as Sly syndrome. One pharmaceutical application for these enzymes is the metabolism of glucuronidated prodrugs into active pharmacological compounds. As expression and activities of β-Glucuronidases vary substantially between tissue types and disease states, these enzymes have been used to achieve targeted activation of oncotherapeutic compounds, some of which may be toxic to healthy cells not associated with malignancy or disease. It is thus important to have knowledge of the β-Glucuronidase activity in the tested sample to determine whether the prodrug or active form will predominate. BioVision’s β-Glucuronidase Activity Assay Kit provides a quick, reliable fluorometric method for measurements of β-Glucuronidase activities of samples and tissue lysates. The provided substrate, which is specific to β-Glucuronidases, is cleaved into a fluorescent product in the presence of β-Glucuronidase. This kit, when used according to the protocol, is sensitive enough to detect as little as one µU (1 pmole/min) of activity. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 330/450 nm) • Species reactivity - N/A• Applications - Measurement of β-Glucuronidase activity in various biological samples and purified/crude enzyme preparations |
| 样本类型以及上样量 | • Adherent and suspension cell lysates• Animal tissues such as rat liver • Purified enzyme |
| 产品特色 | • Simple, rapid & convenient assay to measure β-Glucuronidase • High-throughput adaptable• Includes Positive Control (β-Glucuronidase ) |
| 试剂盒组分 | • β-Glucuronidase Assay Buffer• β-Glucuronidase Substrate• β-Glucuronidase Positive Control• 4-Methylumbelliferone Standard (5 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
