HDAC8 Inhibitor Screening Kit (Fluorometric)
货号：
K368-100 基本售价：
6189.5 元 规格：
100assays
产品信息
概述| 货号 | K368-100 |
| 描述 | Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In BioVision’s HDAC8 Inhibitor screening Kit, HDAC8 deacetylates the substrate (R-H-K(Ac)-K(Ac)-AFC) followed by an enzymatic cleavage to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. In the presence of an HDAC-8 inhibitor, the deacetylation will be impeded which prevents the cleavage of the substrate to release AFC. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput screening of HDAC8 inhibitors. Trichostatin A is included as a control inhibitor to compare the efficacy of the test inhibitors. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Em/Ex = 380/500 nm) • Sample type- Testing candidate HDAC8 inhibitors • Applications- Suitable for high throughput screening of HDAC-8 inhibitors |
| 产品特色 | • Simple procedure; takes ~ 60 min • Fast and convenient • Kit contains the necessary reagents for testing putative HDAC-8 inhibitors |
| 试剂盒组分 | • HDAC8 Assay Buffer • HDAC8 Substrate • HDAC8 Enzyme • Developer • Trichostatin A (2 µM in DMSO) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
