PEP Colorimetric/Fluorometric Assay Kit
货号：
K365-100 基本售价：
6189.5 元 规格：
100assays
产品信息
概述| 货号 | K365-100 |
| 描述 | Phosphoenolpyruvate (PEP) is an important intermediate in carbohydrate metabolism. Containing a high-energy phosphate bond, PEP is involved in glycolysis and gluco-neogenesis and in the shikimate pathway as well as in carbon fixation in plants. Bacteria utilize PEP in the phosphor-transferase system to acquire sugars from the environment. In the glycolytic pathway, PEP is formed from 2-phosphoglycerate by enolase and generates ATP through the action of pyruvate kinase. BioVision’s PEP Assay Kit provides a convenient colorimetric and fluorometric means to measure PEP levels in various samples. In the assay, PEP is converted to ATP and pyruvate. The generated pyruvate is quantified by colorimetric (λmax = 570 nm) or fluorometric methods (Ex/Em = 535/587 nm). The assay is simple, sensitive and reliable. The detection limit is approximately 1 µM PEP in biological samples. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm) • Sample type- Cell/tissue lysates, urine, plasma, serum, and plant extracts • Species reactivity- Mammalian • Applications- The detection limit is approximately 1 µM PEP i |
| 产品特色 | • Simple procedure • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable. |
| 试剂盒组分 | • PEP Assay Buffer • PEP Probe (in DMSO) • PEP Converter • PEP Developer Mix • PEP Standard (1 μmole) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
