JNK Activity Screening Kit, KinaseSTAR
货号：
K430-40 基本售价：
6129.0 元 规格：
40assays
产品信息
概述| 货号 | K430-40 |
| 描述 | c-Jun N-terminal kinase (JNK) is one of the several main MAP kinase groups identified in mammals. Recent evidences suggest that activation of JNK may play an important role in neuronal apoptosis and other physiological and pathological processes. The JNK Activity screening kit utilizes an N-terminal c-Jun (1-79) fusion protein bound to glutathion sepharose beads to selectively “pull down” JNK from cell lysates. After wash to remove nonspecifically bound proteins, the kinase reaction is then carried out in the presence of cold ATP. c-Jun phosphorylation is then measured by Western blot analysis using a phospho-c-Jun specific antibody. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Western blot analysis with anti-Phospho-cJun (Ser 73) Specific Antibody at 1:1000 dilutions • Sample type- Cell and Tissue lysates • Species reactivity- Mammalian • Applications- The kit can be used to screen JNK activity relatively |
| 产品特色 | • Simple procedure • The kit is suitable for both research and drug discovery • The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody. |
| 试剂盒组分 | • Kinase Extraction Buffer • c-Jun (1-79) Fusion Protein Beads • Kinase Assay Buffer • ATP (10 mM) • Phospho-cJun Specific Antibody |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
