Beta-Secretase Activity Fluorometric Assay Kit
货号：
K360-100 基本售价：
6068.5 元 规格：
100assays
产品信息
概述| 货号 | K360-100 |
| 描述 | β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer’s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em 335-355/ 495-510 nm) • Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids • Species reactivity- Mammalian • Applications- Convenient fluorescence |
| 产品特色 | • Simple procedure; takes ~ 1 hour • Fast and convenient • The assay is sensitive, stable and high-throughput adaptable. |
| 试剂盒组分 | • β-Secretase Extraction Buffer • β-Secretase Reaction Buffer (2X) • β-Secretase Substrate (in DMSO) • Active β-Secretase (Lyophilized) • β-Secretase Inhibitor (in DMSO) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
