Total Collagen Assay Kit (Colorimetric)
货号：
K218-100 基本售价：
6068.5 元 规格：
100assays
产品信息
概述| 货号 | K218-100 |
| 描述 | Collagen is the most abundant insoluble protein found in the extracellular matrix and connective tissues. It can be found in skin, tendons, bone, cartilage, muscle, vitreous humor and ligaments, among other tissues. There are more than sixteen - well characterized types of collagens, but types I, II and III collagen comprise more than 80% content in mammals. The triple-helical structure of collagen is quite unique: it consists of a repeating pattern of a basic trimer: Glycine-Proline-Hydroxyproline. In cells, collagens are secreted as procollagens and these chains are transported into the Endoplasmic Reticulum, where, numerous post-translational modifications lead to the formation of a triple helix with disulfide bonds. Excessive production of collagen is linked to pathological conditions including liver cirrhosis, lung fibrosis, and tumor growth. BioVision’s Collagen Assay Kit is a simple and sensitive assay to detect small amounts of collagens in a variety of samples. The assay is based on the acid hydrolysis of samples to form hydrolysates and Hydroxyproline. This released Hydroxyproline gets oxidized to form a reaction intermediate, which further in the reaction, forms a chromophore (Abs 560 nm). The assay is simple, sensitive and specific for collagen and can detect as low as 0.5 µg of collagen in a variety of samples such as tissue homogenates, biological fluids and purified proteins. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Detection method- Absorbance (OD = 560 nm). • Species reactivity - Mammalian tissues, protein/peptide hydrolysates, serum, urine• Applications - Measurement of collagen in various sample types. |
| 样本类型以及上样量 | • |
| 产品特色 | • Simple, fast and convenient assay• Can detect as low as 0.5 µg of collagen in a variety of samples• |
| 试剂盒组分 | • Oxidation Buffer• Chloramine T Concentrate• Perchloric Acid/Isopropanol Solution• DMAB Concentrate (in DMSO)• Collagen I Standard (2 mg/ml) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
