NF-κB (p65) Transcription Factor Assay Kit
货号:
10007889-96Well 基本售价:
5950.0 元 规格:
96 wells
产品信息
概述货号 | 10007889-96Well |
描述 | Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96-well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p65) is detected by addition of specific primary antibody directed against NF-κB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s NF-κB (p65) Transcription Factor Assay detects human NF-κB (p65). It will not cross-react with NF-κB (p50). |
性能供应商 | Cayman |
运输条件 | Dry ice in continental US; may vary elsewhere |
存放说明 | -80 |
稳定性 | ≥ 6 months |
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