PPARγ Transcription Factor Assay Kit
货号：
10006855-96Well 基本售价：
5950.0 元 规格：
96 wells
产品信息
概述| 货号 | 10006855-96Well |
| 描述 | Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.1 The Cayman Chemical PPARγ Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARγ binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARγ is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. |
| 别名 | Peroxisome Proliferator-activated Receptor γ Transcription Factor Assay Kit; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Olefsky, J.M. Nuclear receptor minireview series. The Journal of Biological Chemisty 276(40), 36863-36864 (2001).
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| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 稳定性 | ≥ 6 months |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
