| 货号 | 10006910-96Well |
| 描述 | The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis.1,2 HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.3,4,5 Cayman’s HIF-1α Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysate. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) consensus sequence containing the HIF-1α response element is immobilized to the wells of a 96-well plate. HIF-1α contained in a nuclear extract or whole cell lysates, binds specifically to the HIF-1α response element. The HIF transcription factor complex is detected by addition of a specific primary antibody directed against HIF-1α. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. |
| 别名 | Hypoxia-Inducible Factor-1α; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Wang, G.L.,Jiang, B.H.,Rue, E.A., et al. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proceedings of the National Academy of Sciences of the United States of America 92, 5510-5514 (1995). 2.Safran, M., and Kaelin, W.G., Jr. HIF hydroxylation and the mammalian oxygen-sensing pathway. Journal of Clinical Investigation 111, 779-783 (2003). 3.Rapisarda, A.,Uranchimeg, B.,Scudiero, D.A., et al. Identification of small molecule inhibitors of hypoxia-inducible factor 1 transcriptional activation pathway. Cancer Research 62, 4316-4324 (2002). 4.William, C.,Masson, N.,Tian, Y.M., et al. Peptide blockade of HIFα degradation modulates cellular metabolism and angiogenesis. Proceedings of the National Academy of Sciences of the United States of America 99(16), 10423-10428 (2002). 5.Welsh, S.,Williams, R.,Kirkpatrick, L., et al. Antitumor activity and pharmacodynamic properties of PX-478, an inhibitor of hypoxia-inducible factor-1α. Molecular Cancer Therapeutics 3(3), 233-244 (2004). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 稳定性 | ≥ 6 months |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |