Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit
货号：
K758-100 基本售价：
5947.5 元 规格：
100assays
产品信息
概述| 货号 | K758-100 |
| 描述 | Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In Biovision’s Enteropeptidase Activity Assay Kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em = 380/500 nm) Application: • Measurement of Enteropeptidase activity in biological samples or purified Enteropeptidase activity. • Removing tag from recombinant proteins having recognition motif. |
| 产品特色 | • Simple & convenient procedure.• Can detect enteropeptidase activity as low as 1 mU. • Kit contains all necessary reagents for measuring enteropeptidase activity. |
| 试剂盒组分 | • Enteropeptidase Assay Buffer • Enteropeptidase Substrate (10 mM, in DMSO) • Human Enteropeptidase (Positive Control) • AFC Standard (1 mM) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
