PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay Kit (Fluorometric)
货号：
K248-100 基本售价：
5947.5 元 规格：
100assasys
产品信息
概述| 货号 | K248-100 |
| 描述 | D-2-Hydroxyglutarate Dehydrogenase (D2HGDH: EC 1.1.99.2) is a mitochondrial enzyme which catalyzes the conversion of D-2-Hydroxyglutarate into 2-Oxoglutarate. In humans, mutation of D2HGDH gene could reduce D2HGDH activity which will cause the D-2-Hydroxyglutarate accumulation in body fluids. Increased D-2-Hydroxyglutarate level in body fluids leads to D-2-Hydroxyglutaric Aciduria, a neurometabolic disorder characterized as developmental delay, epilepsy, hypotonia, and dysmorphic features. BioVision’s PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay kit provides a quick and easy method for monitoring D2HGDH activity in a wide variety of samples. In this assay, D2HGDH converts D-2-Hydroxyglutarate into 2-Oxoglutarate and NADH, which further reduces PicoProbe™ to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 4 µU/µl of D2HGDH activity. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorescence (Ex/Em=535/587 nm)• Species reactivity - Mammalian• Applications - Detection of D-2-Hydroxyglutarate Dehydrogenase activity |
| 样本类型以及上样量 | • Purified recombinant protein• Cell and tissue lysate |
| 产品特色 | • Rapid, sensitive and convenient• The kit measures total Activity of D-2-Hydroxyglutatare Dehydrogenase Activity with limit of quantification 4 µU/µl using recombinant D2HGDH |
| 试剂盒组分 | • D2HGDH Assay Buffer• PicoProbe™ (in DMSO)• D2HGDH Substrate• D2HGDH Developer• D2HGDH Positive Control• NADH Standard |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
