| 货号 | 10006270-96Well |
| 描述 | Renin is an aspartyl protease of approximately 40 kDa that is released in active form from renal juxtaglomerular cells in response to sodium depletion, decreased blood volume and blood pressure, and b-adrenergic stimulation.1,2,3 Renin catalyzes the initial and rate limiting step in the renin-angiotensin system (RAS) pathway, converting angiotensinogen into angiotensin I. Angiotensin Converting Enzyme (ACE) subsequently converts angiotensin I to angiotensin II, which is a potent vasoconstrictor. Cayman’s Renin Inhibitor Screening Assay Kit provides a convenient assay in a 96-well format for evaluating human renin inhibitors. The assay utilizes a renin-based synthetic peptide substrate which incorporates the fluorophore EDANS at one end and an EDAN-quenching molecule (Dabcyl) at the other end.4 After cleavage by renin, the peptide-EDANS product is released yielding bright fluorescence which can be easily analyzed using excitation wavelengths of 335-345 nm and emission wavelengths of 485-510 nm. The assay kit includes recombinant human renin (sufficient for 100 reactions), substrate, buffers, and complete instructions. |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Imai, T.,Miyazaki, H.,Hirose, S., et al. Cloning and sequence analysis of cDNA for human renin precursor. Proceedings of the National Academy of Sciences of the United States of America 80, 7405-7409 (1983). 2.Cartledge, S., and Lawson, N. Aldosterone and renin measurements. Annals of Clinical Biochemistry 37, 262-278 (2000). 3.Persson, P.B. Renin: Origin, secretion and synthesis. Journal of Physiology 552.3, 667-671 (2003). 4.Wang, G.T.,Chung, C.C.,Holzman, T.F., et al. A continuous fluorescence assay of renin activity. Analytical Biochemistry 210, 351-359 (1993). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 稳定性 | ≥ 1 year |
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