Hexokinase Colorimetric Assay Kit
货号：
K789-100 基本售价：
5826.5 元 规格：
100assays
产品信息
概述| 货号 | K789-100 |
| 描述 | Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. Early detection of abnormal hexokinase activity is crucial for diagnosis, prediction and treatment of the disease. In BioVision’s Hexokinase Assay kit, glucose is converted to glucose-6-phosphate by hexokinase; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and rapid and can detect hexokinase activity even less than 0.1 mU/well. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Species reactivity- Mammalian • Applications- Measurement of Hexokinase activity in various tissues/cells. Analysis of glucose metabolism and cell signaling in various cell types. Screening anti-diabetic drugs. |
| 样本类型以及上样量 | |
| 产品特色 | • Simple procedure • Sensitive and rapid • Can detect hexokinase activity even less than 0.1 mU/well |
| 试剂盒组分 | • Hexokinase Assay Buffer• HK Substrate• HK Coenzyme (lyophilized)• HK Enzyme Mix (lyophilized)• HK Developer (lyophilized)• NADH Standard (lyophilized)• HK Positive Control (lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
