ADPsensor™ Universal Kinase Activity Assay Kit
货号：
K212-100 基本售价：
5705.5 元 规格：
100assays
产品信息
概述| 货号 | K212-100 |
| 描述 | Protein Kinases belong to a large family of phosphotransferases that mediate a wide spectrum of metabolic events via signal transduction pathways. Protein Kinases catalyze transfer of a phosphate group from a phosphate donor to a substrate protein. Kinases modulate the function of numerous proteins and are well-known therapeutic targets in many diseases like cancer, inflammation and diabetes etc. BioVision’s ADPsensor™ Universal Kinase Assay Kit is based on the fluorescence detection of ADP generated by Kinase activity. In this assay, ADP formed during the kinase reaction is converted to an intermediate by the ADP Sensor Mix, which then reacts with a probe to generate a strong and stable fluorescence signal at Ex/Em = 535/587 nm. ATP in the kinase reactions doesn’t interfere with the assay; therefore our kinase assay can be read kinetically (or at endpoint). The kit provides a universal tool to measure kinase activity in presence of substrates of your interest, or to screen/characterize kinase inhibitors. The assay is simple, non-radioactive, highly sensitive (<0.05 µM ADP) with broad ATP tolerance (up to 1 mM). The kit can detect as low as 1 ng of active Kinase. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Fluorometric (Ex/Em = 535/587 nm)• Species reactivity - Universal• Applications - Detection of Kinase activity, Characterization of Kinase substrates and inhibitors. |
| 样本类型以及上样量 | • Purified/semi-purified/immuno-precipitated Kinases• Crude cell/tissue extract |
| 产品特色 | • Ultrasensitive, Simple, HTP adaptable fluorometric assay kit for Kinase activity measurement.• Can detect as low as 1 ng of active Kinase.• Free of ATP interference ( up to 1 mM) |
| 试剂盒组分 | • Kinase Assay Buffer• ADP Sensor I• ADP Sensor II • Probe (in DMSO)• Ultra-pure ATP (ADP depleted)• DTT (1M)• ADP Standard |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
