Glucose-1-Phosphate Colorimetric Assay Kit
货号：
K697-100 基本售价：
5342.5 元 规格：
100assays
产品信息
概述| 货号 | K697-100 |
| 描述 | Glucose-1-phosphate (G1P) is an important carbohydrate intermediate in glucose metabolism and storage. In response to hormonal or neural signals, glycogenolysis occurs in liver and muscle tissues where Glucose-1-phosphate is released as the rate-limiting step in glycogen breakdown. Glucose-1-phosphate is subsequently converted to Glucose-6-phosphate by phosphoglucomutase and enters glycolysis. In glycogen synthesis, glucose is transferred to glycogen through the actions of Phosphoglucose isomerase (G6P -> G1P), UDPG-pyrophosphorylase (G1P -> UDP-glucose) and glycogen synthase (UDP-glucose + glycogen[n] -> UDP + glycogen[n+1]). Glucose-1-phosphate is found in virtually all organisms from bacteria to higher plants and animals. Measurement of intracellular G1P levels is crucial for analyzing the carbohydrate metabolic pathways and their kinetic properties. In Glucose-1-phosphate assay, G1P is converted to glucose-6-phosphate by phosphoglucomutase in the presence of Glucose 1,6-biphosphate; glucose-6-phosphate is subsequently oxidized by glucose-6-phosphate dehydrogenase to form NADH which reduces a colorless probe to a colored product with strong absorbance at 450 nm. BioVision’s Glucose-1-phosphate assay kit is rapid, sensitive & easy to use & can detect 1µM to 10 mM G1P. This G1P assay kit can be used for a variety of sample types. |
| 克隆号 | BV3-2 |
| 同种亚型 | Mouse IgG2b |
| 来源宿主 | Mouse |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm)• Species reactivity- Mammalian • Applications- The kit can detect glucose-1-phosphate concentrations in the range of 1 µM – 10 mM. |
| 样本类型以及上样量 | Animal tissues: Liver, muscle and heart etc. Cell culture: Adherent or suspension cells. |
| 产品特色 | • Simple procedure; takes ~ 40 minutes• Rapid, convenient and sensitive• Kit contains all necessary reagents for accurate measurement of glucose-1-phosphate levels |
| 试剂盒组分 | • G1P Assay Buffer• G1P Enzyme Mix (Lyophilized)• G1P Developer (Lyophilized)• G1P Substrate Mix (Lyophilized)• G1P Standard (Lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
