Phosphoglucose Isomerase Activity Colorimetric Assay Kit
货号：
K775-100 基本售价：
5221.5 元 规格：
100assays
产品信息
概述| 货号 | K775-100 |
| 描述 | Phosphoglucose isomerase (PGI, EC 5.3.1.9) is an important housekeeping enzyme. PGI catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. PGI performs multiple functions & intracellularly plays key role in both glycolysis and gluconeogenesis. Extracellularly, PGI [also called Autocrine Motility Factor (AMF)] functions as a cytokine, which stimulates cell motility and is associated with tumor development and metastasis. In humans, PGI deficiency causes hemolytic anemia, whereas increased PGI activity is observed in many cancers such as gastrointestinal, kidney and breast cancer. Early detection of abnormal phosphoglucose isomerase activity is crucial for diagnosis, prediction and therapeutic strategy. In BioVision’s Phosphoglucose Isomerase Activity Assay Kit, PGI converts fructose-6-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to give strong absorbance at 450 nm. The PGI assay is simple, sensitive and rapid and can detect phosphoglucose isomerase activity less than 0.1 mU/reaction. |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (450 nm) • Application- The kit can detect phosphoglucose isomerase activity less than 0.1 mU/reaction. |
| 样本类型以及上样量 | • Animal tissues: Liver, brain, heart, kidney etc.• Cell culture: Adherent or suspension cells• Bacteria, yeast, fish etc. |
| 产品特色 | • Simple procedure • Rapid, Convenient and Reliable • Sensitive assay for measuring phosphoglucose isomerase activity in various biological samples |
| 试剂盒组分 | • PGI Assay Buffer • PGI Substrate (Lyophilized) • PGI Enzyme Mix (Lyophilized) • PGI Developer (Lyophilized) • NADH Standard (Lyophilized) • PGI Positive Control (Lyophilized) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
