Bilirubin (Total and Direct) Colorimetric Assay Kit
货号:
K553-100 基本售价:
5221.5 元 规格:
100assays
产品信息
概述货号 | K553-100 |
描述 | Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert’s syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts. BioVision’s Bilirubin Assay Kit utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo- salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of catalyst (550 nm). |
同种亚型 | Rabbit IgG |
来源宿主 | Rabbit |
性能供应商 | Biovision |
检测类型 | Absorbance (600 nm for Total Bilirubin and 550 nm for Direct Bilirubin)• Application- Measurement of Bilirubin in Serum samples |
样本类型以及上样量 | • Biological fluids: Serum |
产品特色 | • Simple protocol, robust and reliable data• This assay allows detection of both Total and Direct or conjugated Bilirubin in serum samples.ell |
试剂盒组分 | • Bilirubin Reagent 1• Bilirubin Reagent 2• Catalyst• Total Bilirubin Probe• Direct Bilirubin Probe• Bilirubin Standard (0.2 µg/µl)• DMSO (Anhydrous) |
运输条件 | Blue Ice |
存放说明 | -20℃ |