Adenylate Kinase (AK) Activity Assay Kit (Colorimetric/Fluorometric)
货号：
K350-100 基本售价：
5221.5 元 规格：
100assays
产品信息
概述| 货号 | K350-100 |
| 描述 | Adenylate Kinase (AK) (EC 2.7.4.3) is an abundant enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. The enzyme is found in the nucleus, cytosol, or mitochondria (intermembrane space or matrix) of various kinds of tissues. Adenylate kinase acts on two molecules of ADP to generate ATP and AMP. Nine isoforms of adenylate kinase have been identified. Erythrocyte adenylate kinase deficiency is associated with hemolytic anemia. Adenylate kinase also plays an important role in post-ischemic recovery and in apoptosis. BioVision’s AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming an end product that can be measured colorimetrically (OD 570 nm) or fluorometrically (Ex/Em = 535/587 nm). |
| 同种亚型 | Rabbit IgG |
| 来源宿主 | Rabbit |
性能| 供应商 | Biovision |
| 检测类型 | Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm)• Applications - Detection of Adenylate Kinase activity |
| 样本类型以及上样量 | • Purified Adenylate Kinase• Cell and tissue lysate• Mitochondrial lysate |
| 产品特色 | • Simple, rapid & convenient• The kit includes all the reagents required to measure AK activity• |
| 试剂盒组分 | • AK Assay Buffer • AK Probe• ADP Substrate• AK Convertor • AK Developer • Positive Control (AK Enzyme)• ATP Standard (1 µmol) |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
